8EDI

Structure of C. elegans UNC-5 IG 1+2 Domains bound to Heparin dp4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	120
Detector technology	PIXEL
Collection date	2021-08-01
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.033167
Spacegroup name	P 61 2 2
Unit cell lengths	69.460, 69.460, 424.990
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.100 - 2.110
R-factor	0.2419
Rwork	0.240
R-free	0.27230
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8edc
RMSD bond length	0.010
RMSD bond angle	1.178
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.100	2.300
High resolution limit [Å]	2.110	2.110
Rmerge	0.059	1.660
Rmeas	0.061	1.720
Rpim	0.014	0.430
Number of reflections	21299	1065
<I/σ(I)>	27	2
Completeness [%]	58.0	24.6
Redundancy	18.4	14.8
CC(1/2)	0.999	0.724

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	295	4% (v/v) Pentaerythritol ethoxylate (3/4 EO/OH), 0.1 M sodium acetate pH 4.6, 16% (w/v) PEG 8000, 0.2 M NaCl