8EDC

Structure of C. elegans UNC-5 IG 1+2 Domains

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	120
Detector technology	CCD
Collection date	2006-09-22
Detector	ADSC QUANTUM 210
Wavelength(s)	0.9795
Spacegroup name	C 2 2 2
Unit cell lengths	41.282, 130.216, 97.549
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.770 - 2.890
R-factor	0.2498
Rwork	0.246
R-free	0.28510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4v2a
RMSD bond length	0.002
RMSD bond angle	0.418
Data reduction software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.20_4444)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.070
High resolution limit [Å]	2.890	2.890
Rmerge	0.104	0.855
Rmeas	0.114	0.948
Number of reflections	6114	894
<I/σ(I)>	14.6	1.89
Completeness [%]	98.6	92.8
Redundancy	5.1	4.3
CC(1/2)	0.997	0.722

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	295	1.25 M (NH4)2SO4, 0.1 M Tris pH 8.5, 0.2 M Li2SO4