8EDB

5'-CGCGAATTCGCG-3' and an AT-specific binder (DB1884) complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-03-07
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	25.770, 39.760, 65.350
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.240 - 1.550
R-factor	0.2012
Rwork	0.200
R-free	0.21570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	IBNA
RMSD bond length	0.013
RMSD bond angle	1.521
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19.2-4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.240	1.600
High resolution limit [Å]	1.550	1.550
Number of reflections	10249	1004
<I/σ(I)>	17.92
Completeness [%]	99.5
Redundancy	2
CC(1/2)	0.990	0.990

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	MPD, SODIUM CACODYLATE TRIHYDRATE, SPERMINE TETRAHYDROCHLORIDE, POTASSIUM CHLORIDE, MAGNESIUM CHLORIDE HEXAHYDRATE