8ECZ
Bovine Fab 4C1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-04-16 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.03321 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 199.058, 97.475, 70.385 |
| Unit cell angles | 90.00, 91.44, 90.00 |
Refinement procedure
| Resolution | 49.750 - 2.820 |
| R-factor | 0.2787 |
| Rwork | 0.243 |
| R-free | 0.25760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6oo0 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.759 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.750 | 2.850 |
| High resolution limit [Å] | 2.820 | 2.820 |
| Rmerge | 0.148 | 1.000 |
| Rmeas | 0.181 | 1.280 |
| Rpim | 0.102 | 0.777 |
| Number of reflections | 29956 | 1238 |
| <I/σ(I)> | 7.2 | 0.8 |
| Completeness [%] | 92.9 | 79.1 |
| Redundancy | 2.7 | 2 |
| CC(1/2) | 0.942 | 0.315 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293.15 | 0.1M Tris, 50% MPD, 0.2M ammonium dihydrogen phosphate |
