8ECQ
Bovine Fab 2G3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-06-26 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.97741 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 66.373, 66.373, 232.768 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.930 - 2.000 |
| R-factor | 0.2161 |
| Rwork | 0.214 |
| R-free | 0.25250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6oo0 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.765 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.930 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.075 | 0.802 |
| Rmeas | 0.077 | 0.911 |
| Rpim | 0.017 | 0.411 |
| Number of reflections | 36241 | 1611 |
| <I/σ(I)> | 29.6 | 1.4 |
| Completeness [%] | 99.2 | 91.2 |
| Redundancy | 16.5 | 3.8 |
| CC(1/2) | 0.993 | 0.549 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277.15 | 10% glycerol, 0.1M Mes, 5% Peg1000, 30% Peg600 |
