8ECH
Tick-borne encephalitis virus capsid protein NLS bound to host importin alpha 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2020-04-29 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 78.209, 90.050, 99.218 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.520 - 2.050 |
| R-factor | 0.1945 |
| Rwork | 0.193 |
| R-free | 0.21910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6bw0 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.548 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.19rc4_4035: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.520 | 2.110 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rpim | 0.039 | 0.330 |
| Number of reflections | 44694 | 3429 |
| <I/σ(I)> | 8.7 | |
| Completeness [%] | 100.0 | |
| Redundancy | 12 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295.15 | 0.7 M sodium citrate, 0.1 M HEPES, pH 6.5 |
