8EBH

Human PU.1 ETS-Domain (165-270) Bound to d(AATAAGCGGAATGGGG)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0000
Spacegroup name	P 1 21 1
Unit cell lengths	42.939, 61.519, 44.394
Unit cell angles	90.00, 116.91, 90.00

Refinement procedure

Resolution	33.290 - 1.330
R-factor	0.1402
Rwork	0.139
R-free	0.17390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8e3k
RMSD bond length	0.008
RMSD bond angle	1.073
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.290	1.378
High resolution limit [Å]	1.330	1.330
Rmerge	0.043	0.446
Rmeas	0.050	0.525
Rpim	0.025	0.271
Number of reflections	46513	4680
<I/σ(I)>	16.72	3.37
Completeness [%]	98.2	99.4
Redundancy	3.8	3.8
CC(1/2)	0.998	0.896

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	2% PEG 3350, 100 mM Sodium Acetate, pH 4.6