8EB9

Crystal structure of SIX8 from Fusarium oxysporum f. sp. lycopersici

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-08-09
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.95373
Spacegroup name	P 43 2 2
Unit cell lengths	51.814, 51.814, 81.599
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.640 - 1.280
R-factor	0.1715
Rwork	0.170
R-free	0.19330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	SIX8 alphafold model
RMSD bond length	0.011
RMSD bond angle	1.210
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.640	1.326
High resolution limit [Å]	1.280	1.280
Rmerge	0.048	1.350
Rmeas	0.050	1.405
Rpim	0.014
Number of reflections	29449	2869
<I/σ(I)>	23.71
Completeness [%]	100.0	99.1
Redundancy	13
CC(1/2)	0.999	0.725

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.17 M Ammonium sulfate, 15% glycerol, 25.5% PEG4000