8EAW

An asymmetric disk assembly formed by tandem dimers of the tobacco mosaic viral capsid protein (TMV)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2019-03-18
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0000
Spacegroup name	C 2 2 21
Unit cell lengths	208.510, 255.500, 260.850
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	81.440 - 2.800
R-factor	0.1975
Rwork	0.196
R-free	0.23450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ei7
RMSD bond length	0.002
RMSD bond angle	0.495
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX (1.18.2_3874)
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	81.440	2.900
High resolution limit [Å]	2.800	2.800
Number of reflections	169799	16816
<I/σ(I)>	9.55
Completeness [%]	99.7	98.82
Redundancy	8.3	8.1
CC(1/2)	0.996	0.467

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	100 mM Bis-Tris, pH 6.5, 45 % (v/v) polypropylene glycol P400