8EA2
Structure of 2-hydroxyisoflavanone dehydratase from Pueraria lobate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-10-08 |
| Detector | RIGAKU |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 102.309, 102.309, 70.239 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.240 - 2.394 |
| R-factor | 0.189 |
| Rwork | 0.186 |
| R-free | 0.24330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2o7r |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.274 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.394 | 2.394 |
| Rmerge | 0.083 | 0.593 |
| Number of reflections | 16673 | 1693 |
| <I/σ(I)> | 14.8 | 2.4 |
| Completeness [%] | 98.0 | 100 |
| Redundancy | 6 | 6.1 |
| CC(1/2) | 0.830 | 0.780 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 18% PEG3350, 0.1 M HEPES pH7.5 |
