8E9P

Crystal structure of wild-type E. coli aspartate aminotransferase in the ligand-free form at 278 K

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	278
Detector technology	PIXEL
Collection date	2021-06-01
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9795
Spacegroup name	P 63
Unit cell lengths	143.830, 143.830, 81.570
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.080 - 2.080
R-factor	0.1774
Rwork	0.174
R-free	0.20550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1x28
RMSD bond length	0.002
RMSD bond angle	0.486
Data reduction software	xia2
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	62.510	2.130
High resolution limit [Å]	2.080	2.090
Rmerge	0.200
Number of reflections	57093	2848
<I/σ(I)>	6.8
Completeness [%]	100.0
Redundancy	10.5
CC(1/2)	0.995	0.227

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	HEPES, ammonium sulfate, PEG 400, maleate