8E8K

Human DNA polymerase eta-DNA-rC-ended primer-dGMPNPP ternary mismatch complex with Mg2+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2022-02-08
Detector	RAYONIX MX-300
Wavelength(s)	0.978720
Spacegroup name	P 61
Unit cell lengths	98.077, 98.077, 81.890
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.470 - 2.400
R-factor	0.1849
Rwork	0.182
R-free	0.23720
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7m7l
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	42.470	42.470	2.520
High resolution limit [Å]	2.380	7.070	2.380
Rmerge	0.057	0.030	0.320
Rmeas	0.060	0.031	0.345
Total number of observations	368412
Number of reflections	34906	1345	5246
<I/σ(I)>	28.43	65.15	5.12
Completeness [%]	98.7	99.6	91.8
Redundancy	10.554	10.976	6.973
CC(1/2)	0.999	1.000	0.945

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	PEG 2000MME, 0.1 M MES