8E7Y

RsTSPO A138F with two heme bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-30
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.033
Spacegroup name	P 21 21 21
Unit cell lengths	40.110, 95.350, 105.550
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.170 - 2.300
R-factor	0.2167
Rwork	0.216
R-free	0.25050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4uc1
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.690	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.125	1.430
Number of reflections	18728	1832
<I/σ(I)>	10.9
Completeness [%]	99.9	100
Redundancy	9.6
CC(1/2)	0.995	0.698

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE		293	28-32% PEG 400, 300mM ammonium formate, 100mM Tris (pH 9.0)