8E7X

RsTSPO A138F with one Heme bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-06-07
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.033
Spacegroup name	C 1 2 1
Unit cell lengths	57.952, 100.103, 95.614
Unit cell angles	90.00, 100.23, 90.00

Refinement procedure

Resolution	44.190 - 2.100
R-factor	0.1979
Rwork	0.196
R-free	0.23240
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4uc1
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.200	2.170
High resolution limit [Å]	2.100	2.100
Rmerge	0.077	0.452
Number of reflections	30027	3066
<I/σ(I)>	4.9	1.4
Completeness [%]	99.0
Redundancy	1.9
CC(1/2)	0.994	0.654

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE		293	28-30% PEG1000, 50mM Sodium Chloride, 20mM Glycine (pH 9.0), 6% 2-Propanol