8E3K
Human PU.1 ETS-Domain (165-270) Bound to d(AATAAGCGGAAGTGGG)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-02-02 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 43.001, 60.742, 44.574 |
Unit cell angles | 90.00, 116.71, 90.00 |
Refinement procedure
Resolution | 33.300 - 1.280 |
R-factor | 0.1282 |
Rwork | 0.127 |
R-free | 0.15120 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1pue |
RMSD bond length | 0.008 |
RMSD bond angle | 1.099 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.300 | 1.326 |
High resolution limit [Å] | 1.280 | 1.280 |
Rmerge | 0.063 | 0.474 |
Rmeas | 0.074 | 0.551 |
Rpim | 0.038 | 0.276 |
Number of reflections | 51827 | 5190 |
<I/σ(I)> | 11.84 | 2.32 |
Completeness [%] | 98.1 | 98.65 |
Redundancy | 3.8 | 3.8 |
CC(1/2) | 0.992 | 0.896 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 2% PEG 3350, 100 mM Sodium Acetate, pH 4.6 |