8E3E

ZBTB7A Zinc Finger Domain Bound to DNA Duplex Containing CAST sequence (#10)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-06-09
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00000
Spacegroup name	P 1 21 1
Unit cell lengths	67.519, 147.175, 68.064
Unit cell angles	90.00, 96.46, 90.00

Refinement procedure

Resolution	49.800 - 2.990
R-factor	0.2047
Rwork	0.203
R-free	0.23540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7n5u
RMSD bond length	0.003
RMSD bond angle	0.514
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.800	3.100
High resolution limit [Å]	2.990	2.990
Rmerge	0.080	0.420
Rpim	0.044	0.331
Number of reflections	26193	2577
<I/σ(I)>	15.7	2.1
Completeness [%]	98.7	97.6
Redundancy	7.1	6.4
CC(1/2)		0.945

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	292	12% PEG3350, 0.1M Sodium malonate pH7.0