8E37

Structure of Campylobacter concisus wild-type SeMet PglC

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-11-30
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.0
Spacegroup name	P 31 2 1
Unit cell lengths	142.820, 142.820, 192.563
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.990 - 3.010
R-factor	0.2672
Rwork	0.266
R-free	0.29580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5W7L
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.700	3.121
High resolution limit [Å]	3.010	3.010
Rmeas	0.105
Number of reflections	38779	3544
<I/σ(I)>	14.2
Completeness [%]	82.8
Redundancy	1.9
CC(1/2)	0.980	0.553

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		290.15	0.1 M Bis-Tris pH 6.0, 0.3 M MgCl2, 27% PEG 3350,1 mM TCEP