8E18
Crystal structure of apo TnmK1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-11-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 86.006, 86.006, 132.392 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.640 - 1.140 |
| R-factor | 0.1336 |
| Rwork | 0.133 |
| R-free | 0.15260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5uno |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.277 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MrBUMP |
| Refinement software | REFMAC (5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.150 |
| High resolution limit [Å] | 1.130 | 1.130 |
| Number of reflections | 172050 | 9157 |
| <I/σ(I)> | 56.6 | |
| Completeness [%] | 100.0 | |
| Redundancy | 9.9 | |
| CC(1/2) | 0.997 | 0.723 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 0.1 M HEPES, pH 7.0, 1.0 M succinic acid, pH 7.0, and 1% w/v PEG 2000 MME |
