8E12
Homotrimeric variant of tcTRP9, BGL14
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-04-15 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.976 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 59.330, 59.330, 260.327 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.790 - 3.000 |
R-factor | 0.2844 |
Rwork | 0.281 |
R-free | 0.32140 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | computationally generated |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER (2.8.3) |
Refinement software | PHENIX (1.20rc1_4392) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.050 |
High resolution limit [Å] | 3.000 | 8.130 | 3.000 |
Rmerge | 0.114 | 0.071 | 0.730 |
Rmeas | 0.117 | 0.073 | 0.807 |
Rpim | 0.028 | 0.019 | 0.327 |
Total number of observations | 163757 | ||
Number of reflections | 10242 | 691 | 261 |
<I/σ(I)> | 6.8 | ||
Completeness [%] | 88.3 | 100 | 46.6 |
Redundancy | 16 | 14.8 | 5.3 |
CC(1/2) | 0.998 | 0.726 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 15% (w/v) PEG 3350, 0.1 M Magnesium formate dihydrate |