8DZV

Chicken anti-cardiac Troponin I antibody in complex with peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2015-09-30
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.953689
Spacegroup name	P 2 21 21
Unit cell lengths	45.800, 74.210, 75.430
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.720 - 1.200
R-factor	0.1615
Rwork	0.161
R-free	0.17360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4p48
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.720	1.243
High resolution limit [Å]	1.200	1.200
Rmerge	0.034	0.162
Rmeas	0.048	0.229
Rpim	0.034	0.162
Number of reflections	80289	8013
<I/σ(I)>	14.2
Completeness [%]	99.0	88.78
Redundancy	1.9
CC(1/2)	0.998	0.925

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293.15	0.1 M MES pH 6.5, 0.2 M potassium iodide, 25% PEG 4000