8DY3

Crystal Structure of spFv GLK2 HL

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0000
Spacegroup name	P 41 21 2
Unit cell lengths	63.030, 63.030, 124.960
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	56.276 - 1.509
R-factor	0.1844
Rwork	0.183
R-free	0.20940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3na9
RMSD bond length	0.007
RMSD bond angle	0.923
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	56.276	1.654
High resolution limit [Å]	1.509	1.509
Rmerge	0.093	1.678
Rmeas	0.097	1.777
Rpim	0.027	0.568
Number of reflections	30532	1527
<I/σ(I)>	17.3
Completeness [%]	94.2	68.8
Redundancy	12.5	9.2
CC(1/2)	0.999	0.513

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	4.5	298	100 mM Na Acetate, 750 mM (NH4)2 SO4