8DY2
Crystal Structure of spFv GLK1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-04-17 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.630, 78.940, 257.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.860 - 1.650 |
| R-factor | 0.2166 |
| Rwork | 0.215 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3na9 |
| Data reduction software | DENZO |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18_3855) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.860 | 1.836 |
| High resolution limit [Å] | 1.648 | 1.648 |
| Rmerge | 0.088 | 0.955 |
| Rmeas | 0.096 | 1.052 |
| Rpim | 0.431 | |
| Number of reflections | 55333 | 2712 |
| <I/σ(I)> | 13.24 | 2 |
| Completeness [%] | 97.2 | 75.8 |
| Redundancy | 6.466 | 5.4 |
| CC(1/2) | 0.999 | 0.667 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.5 | 298 | 0.1 M Na Acetate pH 4.5, 18% PEG 3350, 1 M LiCL |
