8DXV
[T:Au+:T--(pH11-pH7; 5s)] Metal-mediated DNA base pair in tensegrity triangle grown at pH 11 and soaked in pH 7 for 5s
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-12-12 |
| Detector | DECTRIS EIGER2 X 9M |
| Wavelength(s) | 1.00743 |
| Spacegroup name | H 3 |
| Unit cell lengths | 107.175, 107.175, 91.374 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.380 - 4.590 |
| R-factor | 0.1858 |
| Rwork | 0.184 |
| R-free | 0.23840 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.190 |
| Data reduction software | autoPROC |
| Data scaling software | STARANISO |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.120 | 5.280 |
| High resolution limit [Å] | 4.590 | 4.590 |
| Number of reflections | 2862 | 408 |
| <I/σ(I)> | 9 | |
| Completeness [%] | 85.2 | |
| Redundancy | 7.5 | |
| CC(1/2) | 0.999 | 0.560 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 11 | 298 | 40 mM Tris, 125 mM magnesium sulfate, 12 uM gold(I) phosphine |
