8DVQ
CA domain of VanSA histidine kinase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS-II BEAMLINE 17-ID-1 |
Synchrotron site | NSLS-II |
Beamline | 17-ID-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-04-03 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.92009 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 69.830, 94.400, 29.770 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.690 - 2.190 |
R-factor | 0.192 |
Rwork | 0.190 |
R-free | 0.23620 |
Structure solution method | SAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.261 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX (1.14_3260) |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.700 | 2.270 |
High resolution limit [Å] | 2.190 | 2.190 |
Rmerge | 0.090 | |
Rmeas | 0.096 | |
Rpim | 0.033 | |
Number of reflections | 10676 | 1031 |
<I/σ(I)> | 13 | |
Completeness [%] | 99.7 | 98 |
Redundancy | 8.55 | |
CC(1/2) | 0.999 | 0.849 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 8.2 | 291 | Protein and precipitant were mixed in a volume ratio of 1:2 and incubated under Als Oil at 291K; the precipitant solution was obtained from the Berkeley Screen and contained 75 mM Bis-Tris Propane, 25 mM citric acid, pH 8.2, 20 mM CdCl2, and 25 (w-v) PEG 400. |