8DV6
Zika virus envelope protein structure in complex with a potent Human mAb
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-11-03 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 95.041, 133.609, 105.109 |
| Unit cell angles | 90.00, 106.49, 90.00 |
Refinement procedure
| Resolution | 45.570 - 3.380 |
| R-factor | 0.2336 |
| Rwork | 0.232 |
| R-free | 0.25860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5jhm |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.619 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.460 |
| High resolution limit [Å] | 3.380 | 3.380 |
| Rmerge | 0.177 | 1.434 |
| Rpim | 0.070 | 0.585 |
| Number of reflections | 35195 | 1718 |
| <I/σ(I)> | 12.9 | 1.4 |
| Completeness [%] | 99.4 | 100 |
| Redundancy | 7.5 | 7 |
| CC(1/2) | 0.620 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | PEG8000, HEPES |
