8DRG

Structure of Rv0455c from Mycobacterium tuberculosis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-03-27
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	C 1 2 1
Unit cell lengths	70.039, 25.228, 58.631
Unit cell angles	90.00, 104.25, 90.00

Refinement procedure

Resolution	33.966 - 1.800
Rwork	0.157
R-free	0.21090
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8dqy
RMSD bond length	0.006
RMSD bond angle	1.380
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0352)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.966	1.840
High resolution limit [Å]	1.800	1.800
Rmerge		0.614
Number of reflections	9253	544
<I/σ(I)>	7.7
Completeness [%]	97.8
Redundancy	3.4
CC(1/2)	0.968

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	35 % Tacsimate pH 7.0