8DQU

Nanobody bound SARS-CoV-2 Nsp9

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-02-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	P 6
Unit cell lengths	165.025, 165.025, 46.002
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.180 - 2.450
R-factor	0.204449597035
Rwork	0.203
R-free	0.22887
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7kri
RMSD bond length	0.021
RMSD bond angle	1.502
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.600	2.580
High resolution limit [Å]	2.450	2.450
Rpim	0.042	0.660
Number of reflections	25484	3715
<I/σ(I)>	10.6	1.3
Completeness [%]	95.3
Redundancy	3.6
CC(1/2)	0.998	0.409

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	1.8M ammonium sulfate, 0.1M MES pH 6.0