8DQA
Structure of A. thaliana MIF/D-DT-like protein-3 (MDL3)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-03-06 |
| Detector | DECTRIS EIGER2 S 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 89.499, 89.499, 89.499 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.750 - 2.000 |
| R-factor | 0.1962 |
| Rwork | 0.195 |
| R-free | 0.22460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7mrv |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.481 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.18_3845: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.750 | 2.072 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 8229 | 804 |
| <I/σ(I)> | 35.38 | 4.35 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 12.8 | 12.2 |
| CC(1/2) | 1.000 | 0.923 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 50 mM MES, pH 6.0, 4% MPD, 0.2 M ammonium acetate, 30% PEG3350 |
