8DQ2
X-ray crystal structure of Hansschlegelia quercus lanmodulin (LanM) with lanthanum (III) bound at pH 7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2022-03-29 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97857 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.107, 108.658, 44.697 |
| Unit cell angles | 90.00, 90.02, 90.00 |
Refinement procedure
| Resolution | 40.110 - 1.800 |
| R-factor | 0.1894 |
| Rwork | 0.190 |
| R-free | 0.20880 |
| Structure solution method | SAD |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.865 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmeas | 0.133 | 1.130 |
| Rpim | 0.052 | 0.531 |
| Number of reflections | 41911 | 1053 |
| <I/σ(I)> | 12.9 | 1.39 |
| Completeness [%] | 86.7 | 15.2 |
| Redundancy | 6 | 4.2 |
| CC(1/2) | 0.995 | 0.612 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 294 | 0.01 M tri-sodium citrate, 27 % (w/v) PEG 6000 |
