8DPQ
Beta-lactamase CTX-M-14 N170A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2020-02-28 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.11 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 41.418, 41.418, 230.493 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.400 - 1.670 |
| R-factor | 0.17383 |
| Rwork | 0.171 |
| R-free | 0.23292 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ylt |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.171 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.400 | 1.730 |
| High resolution limit [Å] | 1.670 | 1.671 |
| Rmeas | 0.071 | 0.180 |
| Number of reflections | 27171 | 2567 |
| <I/σ(I)> | 9 | |
| Completeness [%] | 97.3 | |
| Redundancy | 9.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.1 M Sodium HEPES pH7.5, 25% (w/v) PEG 8000 |
