8DPK

structure of T. brucei RESC5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-18
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	45.606, 80.441, 76.076
Unit cell angles	90.00, 102.73, 90.00

Refinement procedure

Resolution	54.540 - 1.940
R-factor	0.2226
Rwork	0.221
R-free	0.25860
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	AutoSol
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.540	2.010
High resolution limit [Å]	1.940	1.940
Rpim	0.044	0.228
Number of reflections	38130	2269
<I/σ(I)>	7.4	1
Completeness [%]	99.1
Redundancy	3
CC(1/2)	0.994	0.673

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	60% Tascimate, pH 7.0