8DP8

Crystal structure of the monomeric AvrM14-A Nudix hydrolase effector from Melampsora lini

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2014-06-14
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9537
Spacegroup name	P 21 21 21
Unit cell lengths	37.645, 166.842, 62.911
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.711 - 2.300
R-factor	0.186710724587
Rwork	0.183
R-free	0.25146
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	0.982
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.711	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.160	0.804
Number of reflections	18412	1793
<I/σ(I)>	10.5
Completeness [%]	99.8
Redundancy	5.9
CC(1/2)	0.993	0.721

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	291	2.4 M sodium malonate pH 6.0 2% Glycerol