8DP6

Crystal structure of Helicobacter pylori EgtU

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	80
Detector technology	PIXEL
Collection date	2021-06-12
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	C 1 2 1
Unit cell lengths	168.463, 58.395, 61.886
Unit cell angles	90.00, 109.54, 90.00

Refinement procedure

Resolution	39.530 - 1.300
Rwork	0.170
R-free	0.18030
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	AutoSol
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.530	1.320
High resolution limit [Å]	1.300	1.300
Rpim	0.069	0.952
Number of reflections	137306	13237
<I/σ(I)>	8.4	2.1
Completeness [%]	99.1	84.8
Redundancy	4.5
CC(1/2)	0.994	0.324

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.8	291	0.3 M ammonium sulfate, 22% PEG4000, 0.2 M sodium acetate, pH 4.8