8DOD
Beta-lactamase CTX-M-14 S130A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-09-11 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.11 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 41.490, 41.490, 230.780 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 35.504 - 1.610 |
R-factor | 0.2242 |
Rwork | 0.222 |
R-free | 0.25870 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ylt |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.504 | 1.668 |
High resolution limit [Å] | 1.610 | 1.610 |
Rmerge | 0.014 | 0.063 |
Rmeas | 0.020 | 0.094 |
Number of reflections | 62179 | 6092 |
<I/σ(I)> | 20.94 | |
Completeness [%] | 99.5 | 99.25 |
Redundancy | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.1 M Tris-HCl pH 8.5, 25% (W/V) PEG6000 |