8DN6

The crystal structure of the Arabidopsis thaliana Toc75 POTRA domains in complex with fab tc2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-16
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.03
Spacegroup name	C 1 2 1
Unit cell lengths	276.089, 51.481, 172.082
Unit cell angles	90.00, 123.06, 90.00

Refinement procedure

Resolution	49.300 - 3.000
R-factor	0.2423
Rwork	0.240
R-free	0.29300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5bjz 5uay
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.20_4459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.110
High resolution limit [Å]	3.000	3.000
Rmeas	0.347
Rpim	0.138	0.682
Number of reflections	41871	4117
<I/σ(I)>	7.28	1.21
Completeness [%]	99.9	100
Redundancy	6.2	6.1
CC(1/2)	0.982	0.370

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	16% PEG 4000, 20% Glycerol, 0.1M Sodium Citrate pH 5.8, 0.1M Ammonium Sulphate