8DMP

Crystal structure of Legionella pneumophila macrodomain effector MavL

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-29
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	93.232, 157.244, 117.621
Unit cell angles	90.00, 96.20, 90.00

Refinement procedure

Resolution	39.310 - 2.170
R-factor	0.1876
Rwork	0.187
R-free	0.21420
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6omi
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.310	2.248
High resolution limit [Å]	2.170	2.170
Number of reflections	176902	17628
<I/σ(I)>	11.93
Completeness [%]	99.5
Redundancy	4.8
CC(1/2)	0.995	0.559

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M sodium citrate, 18% w/v PEG 3350