8DLC
Crystal structure of chalcone-isomerase like protein from Vitis vinifera (VvCHIL)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-11-24 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 49.420, 49.420, 170.851 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.800 - 1.900 |
| R-factor | 0.2047 |
| Rwork | 0.201 |
| R-free | 0.23700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4dok |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.103 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.800 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.190 | |
| Rpim | 0.050 | 0.439 |
| Number of reflections | 19994 | 2818 |
| <I/σ(I)> | 10.7 | 2.2 |
| Completeness [%] | 100.0 | |
| Redundancy | 15.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277.15 | 20% (w/v) PEG 3350 and 200 mM potassium nitrate |
