8DJH

Ternary complex of SUMO1 with a phosphomimetic SIM of PML and zinc

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	298
Detector technology	PIXEL
Collection date	2018-09-25
Detector	RIGAKU
Wavelength(s)	1.542
Spacegroup name	P 21 21 21
Unit cell lengths	37.776, 47.460, 63.632
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.040 - 1.770
R-factor	0.2159
Rwork	0.213
R-free	0.24290
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6v7p
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.040	1.835
High resolution limit [Å]	1.770	1.770
Number of reflections	19762	972
<I/σ(I)>	12.02
Completeness [%]	97.2
Redundancy	3
CC(1/2)	0.996	0.750

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	100 mM HEPES pH 7.0, 19 to 31 % (w/v) PEG 3350, and 1-5 mM zinc chloride