8DJ8
Crystal Structure of Calgreen 1 protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-08-01 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97941 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 81.156, 81.156, 84.977 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.620 - 2.000 |
| R-factor | 0.1716 |
| Rwork | 0.169 |
| R-free | 0.19780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2a46 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.090 | 0.668 |
| Number of reflections | 19593 | 839 |
| <I/σ(I)> | 10.1 | 1.403 |
| Completeness [%] | 98.0 | 77 |
| Redundancy | 12.57 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.1 M Sodium citrate, pH 5.6 20% (v/v) 2-Propanol |
