8DI7
CMY-2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2020-12-18 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 55.939, 75.374, 97.552 |
| Unit cell angles | 81.19, 87.84, 69.51 |
Refinement procedure
| Resolution | 61.040 - 1.890 |
| R-factor | 0.2004 |
| Rwork | 0.200 |
| R-free | 0.23910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zc2 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.040 | 1.958 |
| High resolution limit [Å] | 1.890 | 1.890 |
| Rmerge | 0.267 | |
| Rmeas | 0.337 | |
| Rpim | 0.202 | |
| Number of reflections | 113721 | 11281 |
| <I/σ(I)> | 1 | |
| Completeness [%] | 96.3 | 94.56 |
| Redundancy | 20 | |
| CC(1/2) | 1.000 | 0.960 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 293 | 300 mM Ammonium sulfate, 20% PEG 4000 |
