8DHT
Crystal structure of a typeIII Rubisco
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-05-12 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9775 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 80.329, 116.206, 83.285 |
| Unit cell angles | 90.00, 96.32, 90.00 |
Refinement procedure
| Resolution | 49.377 - 1.699 |
| R-factor | 0.1442 |
| Rwork | 0.144 |
| R-free | 0.17740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3kdo |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.730 |
| High resolution limit [Å] | 1.699 | 1.699 |
| Rmerge | 0.062 | 0.377 |
| Rmeas | 0.074 | 0.452 |
| Rpim | 0.040 | 0.247 |
| Number of reflections | 166579 | 8243 |
| <I/σ(I)> | 16.5 | 2.087 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 3.4 | 3.3 |
| CC(1/2) | 0.993 | 0.836 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | 26% PEG4000 (w/v), 0.2M NaAc, 0.1M Tris-HCl, pH8.0 |
