8DHL

Tannerella forsythia beta-glucuronidase (L2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-04
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.96802
Spacegroup name	P 21 21 21
Unit cell lengths	77.983, 119.209, 209.883
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	78.770 - 2.300
R-factor	0.1704
Rwork	0.170
R-free	0.21030
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5uj6
Data reduction software	XDS
Data scaling software	Aimless (0.7.7)
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4489)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	103.660	103.660	2.340
High resolution limit [Å]	2.300	12.390	2.300
Rmerge	0.193	0.119	0.563
Rmeas	0.210	0.133	0.610
Rpim	0.081	0.057	0.234
Total number of observations	575179	3891	29550
Number of reflections	87551	673	4405
<I/σ(I)>	7	10.7	3.3
Completeness [%]	99.9	99.8	100
Redundancy	6.6	5.8	6.7
CC(1/2)	0.979	0.985	0.859

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.8	293.15	6% (w/v) PEG8000, 8% (v/v) ethylene glycol, 2% (v/v) glycerol, 0.1 M Tris-HCl