8DFY
Crystal structure of Human BTN2A1 Ectodomain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-09-29 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9537 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 237.013, 94.189, 134.719 |
| Unit cell angles | 90.00, 106.34, 90.00 |
Refinement procedure
| Resolution | 49.750 - 3.552 |
| R-factor | 0.2559 |
| Rwork | 0.255 |
| R-free | 0.26790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4hh8 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.990 |
| Data reduction software | MOSFLM |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.4 (11-DEC-2020)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.800 | 3.890 |
| High resolution limit [Å] | 3.550 | 3.550 |
| Rpim | 0.108 | 0.513 |
| Number of reflections | 22300 | 1115 |
| <I/σ(I)> | 5.8 | 2 |
| Completeness [%] | 85.2 | 70 |
| Redundancy | 4.6 | 5 |
| CC(1/2) | 0.980 | 0.640 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 1.65 M ammonium sulfate, 2% v/v PEG 400, 0.1 M HEPES |
