8DEN
Heme-Free Cytochrome Variant ApoCyt
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-11-05 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.954 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.590, 85.880, 47.570 |
| Unit cell angles | 90.00, 92.49, 90.00 |
Refinement procedure
| Resolution | 47.530 - 1.690 |
| R-factor | 0.186 |
| Rwork | 0.184 |
| R-free | 0.21660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 8del |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.868 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.17) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.530 | 1.720 |
| High resolution limit [Å] | 1.690 | 1.690 |
| Rmerge | 0.087 | 0.627 |
| Rmeas | 0.095 | 0.689 |
| Rpim | 0.037 | 0.279 |
| Number of reflections | 37769 | 1569 |
| <I/σ(I)> | 10.9 | 2.3 |
| Completeness [%] | 95.0 | 76.7 |
| Redundancy | 6.2 | 5.7 |
| CC(1/2) | 0.998 | 0.731 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 22% PEG 2000, 200 mM MgCl2, 100 mM Tris pH 8.5 |
