8DEI
Structure of the Cac1 KER domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-03-30 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.586, 165.249, 116.149 |
| Unit cell angles | 90.00, 96.47, 90.00 |
Refinement procedure
| Resolution | 28.680 - 2.810 |
| R-factor | 0.238 |
| Rwork | 0.233 |
| R-free | 0.28250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1peb |
| RMSD bond length | 0.001 |
| RMSD bond angle | 0.342 |
| Data reduction software | CrysalisPro |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.680 | 2.910 |
| High resolution limit [Å] | 2.810 | 2.810 |
| Number of reflections | 48320 | 13259 |
| <I/σ(I)> | 7.3 | |
| Completeness [%] | 99.0 | 90.9 |
| Redundancy | 3.9 | 3 |
| CC(1/2) | 0.979 | 0.695 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 288 | 0.1 M Phosphate/citrate pH 4.2 and 30 % PEG 300 |
