8DEG
Crystal structure of DLK in complex with inhibitor DN0011197
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2019-04-25 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97853 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.837, 39.141, 62.781 |
| Unit cell angles | 90.00, 106.99, 90.00 |
Refinement procedure
| Resolution | 30.430 - 2.790 |
| R-factor | 0.2508 |
| Rwork | 0.248 |
| R-free | 0.29660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Not public |
| RMSD bond length | 0.002 |
| RMSD bond angle | 1.225 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.430 | 2.900 |
| High resolution limit [Å] | 2.790 | 2.800 |
| Rmerge | 0.121 | 0.399 |
| Number of reflections | 6597 | 539 |
| <I/σ(I)> | 8.9 | 2.3 |
| Completeness [%] | 96.3 | 81.2 |
| Redundancy | 3.1 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.7 | 293 | 0.2M Magnesium Acetate, 0.1M HEPES pH 7.7, 18% PEG 3350 |
