8DCO
Crystal structure of the GDP-D-glycero-4-keto-D-lyxo-heptose-3,5-epimerase from Campylobacter jejuni, serotype HS:42
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SEALED TUBE |
Source details | BRUKER D8 QUEST |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-12-10 |
Detector | Bruker PHOTON II |
Wavelength(s) | 1.5418 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 73.609, 73.609, 152.346 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.830 - 1.900 |
R-factor | 0.2069 |
Rwork | 0.204 |
R-free | 0.25400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7m14 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.656 |
Data reduction software | SAINT |
Data scaling software | SADABS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.950 |
High resolution limit [Å] | 1.900 | 1.900 |
Number of reflections | 33405 | 2225 |
<I/σ(I)> | 17.6 | 3.4 |
Completeness [%] | 98.7 | 96.5 |
Redundancy | 9.6 | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | Protein incubated with 5 mM GDP; precipitant: 7-12% PEG8000, 200 mM potassium chloride, 100 mM MES, pH 7.0 |