8DCJ

[A:T] Self-Assembled 3D DNA Rhombohedral Tensegrity Triangle

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-21
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	1.00743
Spacegroup name	H 3
Unit cell lengths	105.527, 105.527, 96.025
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.100 - 3.330
R-factor	0.2097
Rwork	0.208
R-free	0.22990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5w6w
RMSD bond length	0.009
RMSD bond angle	1.083
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	66.200	3.660
High resolution limit [Å]	3.330	3.330
Number of reflections	3608	194
<I/σ(I)>	13.3
Completeness [%]	83.2
Redundancy	10.7
CC(1/2)	1.000	0.340

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9.5	293	1.75 M ammonium sulfate, 120 mM Tris, 120 mM Acetic Acid, 6 mM EDTA