8DB6
Adenosine/guanosine nucleoside hydrolase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-1 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-04-07 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.977 |
| Spacegroup name | P 1 |
| Unit cell lengths | 50.596, 75.115, 85.209 |
| Unit cell angles | 76.16, 81.49, 88.14 |
Refinement procedure
| Resolution | 29.650 - 2.020 |
| R-factor | 0.2053 |
| Rwork | 0.204 |
| R-free | 0.23710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | AlphaFold model |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.951 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.650 | 2.092 |
| High resolution limit [Å] | 2.020 | 2.020 |
| Rmerge | 0.085 | 0.613 |
| Rmeas | 0.100 | 0.717 |
| Rpim | 0.052 | 0.369 |
| Number of reflections | 76440 | 6752 |
| <I/σ(I)> | 8.5 | 1.6 |
| Completeness [%] | 96.8 | 85.72 |
| Redundancy | 3.6 | 3.6 |
| CC(1/2) | 0.994 | 0.707 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 290 | 0.2 M ammonium sulfate, 25% PEG-3350, and 0.1 M Bis-tris, pH 5.5 |
