8DB5
Crystal structure of the GDP-D-glycero-4-keto-d-lyxo-heptose-3,5-epimerase from Campylobacter jejuni, serotype HS:15
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | BRUKER D8 QUEST |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-07-21 |
| Detector | Bruker PHOTON II |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.549, 154.525, 120.506 |
| Unit cell angles | 90.00, 91.28, 90.00 |
Refinement procedure
| Resolution | 36.810 - 1.900 |
| R-factor | 0.1913 |
| Rwork | 0.189 |
| R-free | 0.23570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7m14 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.613 |
| Data reduction software | SAINT |
| Data scaling software | SADABS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rpim | 0.081 | |
| Number of reflections | 118325 | 8051 |
| <I/σ(I)> | 9.3 | 2.5 |
| Completeness [%] | 99.3 | 96.9 |
| Redundancy | 4.5 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | Enzyme pre-incubated with 5 mM GDP; precipitant: 26-28% PEG8000, 100 mM MES |
